Long-Read Sequencing in Uppsala

Preliminary program - LRUA 2022

Day 1 (October 31, 2022) – Uppsala University Main Building

08:30 – 10:30 Registration and coffee

*** LONG-READ SEQUENCING UPDATES

10:30 - 10:35 Welcome to LRUA22, Olga Vinnere Pettersson

10:35 - 10:50 Long-read sequencing at SciLifeLab NGI, Adam Ameur

10:50 - 11:15 Updates from ONT, Tonya McSherry, Global Director New Markets

11:15 - 11:40 Updates from PacBio, Jonas Korlach, Chief Scientific Officer

*** BIODIVERSITY

11:40 - 12:10 TBA, Mark Blaxter, Wellcome Sanger Institute, UK

12:10 - 13:30 Lunch & posters

*** BIODIVERSITY Cont.

13:30 - 13:50 Genomic analyses of the Linum distyly supergene reveal convergent

evolution at the molecular level, Tanja Slotte, Stockholm university, Sweden

13:50 - 14:10 TBA, Camila Mazzoni, IZW, Berlin, Germany

14:10 - 14:30 Chromosome-scale assemblies of Norway spruce and Scots pine, Nathaniel

Street, Umeå Plant Science Centre, Umeå University, Sweden

14:30 - 14:50 TBA, Valentina Peona, Uppsala university, Sweden

14:50 - 15:00 Silver sponsor

15:00 - 15:40 Coffee & posters

*** WHAT’S NEXT IN LONG-READ SEQUENCING?

15:40 - 16:10 Streamlining clinical genetic testing: the promise of long-read sequencing, Danny Miller, University of Washington, USA

16:10 - 16:30 Single-cell Analysis of Alternative Splicing in Hematopoietic Stem and Progenitor Cells, Laura Mincarelli, CRUK Beatson Institute, UK

16:30 - 16:50 Single-cell transcript isoform sequencing of the activated adult mouse hippocampus, Sheridan Cavalier, Johns Hopkins School of Medicine, USA

16:50 - 17:10 Long-read sequencing techniques in study of somatic transposition in the Drosophila intestine, Natalia Rubanova, Institut Curie, Paris, France

18.30 – Late Conference dinner (Norrlands Nation)

https://www.lrua2022.se/

Day 2 (November 1, 2022) – Uppsala University Main Building

*** TECHNOLOGY & APPLICATIONS - COMPUTATIONAL APPROACHES

09:00 - 09:30 TBA, Ana Conesa, National Research Council, Spain

09:30 - 09:50 Advanced approaches for chromosome-scale haplotype-resolved assembly and pangenomics, Shilpa Garg, University of Copenhagen, Denmark

09:50 - 10:10 Verkko: telomere-to-telomere assembly of diploid chromosomes, Mikko

Rautiainen, National Institutes of Health USA & FIMM Finland

10:10 - 10:20 Silver sponsor

10:20 - 11:00 Coffee & posters

*** HUMAN/MEDICAL SEQUENCING

11:00 - 11:30 Recent progress in human genome analysis using Nanopore long reads, Miten Jain, Northeastern University, Boston, USA

11:30 - 11:50 Comprehensive human brain transcriptome characterisation using long-read Capture Sequencing, Sofia Kudasheva, UK

11:50 - 12:10 Quantifying Genome-Editing Outcomes Using Long-Read Sequencing and Beyond, Ayal Hendel, Bar-Ilan University, Israel

12:10 - 12:20 Silver sponsor

12:20 - 13:30 Lunch & posters

*** HUMAN/MEDICAL SEQUENCING Cont.

13:30 - 14:00 Long-read genomics for rare diseases, Alexander Hoischen, Radboud University Medical Center, Nijmegen, The Netherlands

14:00 - 14:20 Application of nanopore sequencing for liquid biopsy analysis, Carolin Sauer, EMBL-EBI, UK

14:20 - 14:40 Transcriptome variation in human tissues uncovered by long-read

sequencing, Tuuli Lappalainen, SciLifelab/KTH Sweden & New York Genome Center USA

14:40 - 15:00 Long-read sequencing reveals novel transcripts induced by misexpression of

DUX4 in FSHD muscle, Dongxu Zheng, The Netherlands

15:00 - 15:10 Silver sponsor

15:10 - 15:50 Coffee & posters

*** TECHNOLOGY & APPLICATIONS - CHALLENGES

15:50 - 16:10 TBA, Melissa Laird Smith, University of Louisville, USA

16:10 - 16:30 TBA, Andreas Wallberg, Uppsala university, Sweden

16:30 - 16:50 Long-read sequencing assay allows accurate characterization of the HIV-1

reservoir, Laurens Lambrechts, Ghent University, Belgium

16:50 - 17:10 TBA, Maliheh Mershad, SLU, Sweden

17:10 - 17:30 Genomic signatures of single Platynothrus peltifer from chromosome-scale

genome assembly with resolved haplotypes, Shan Gao, University of Cologne, Germany

Day 3 (November 3, 2022) – Uppsala University Main Building

09:00 - 10:30 Workshop - Oxford Nanopore Technologies

10:30 - 11:00 Coffee

11:00 - 12:30 Workshop - PacBio

12:30 - 13:30 Lunch (on your own)

13.30 - 16.00 Satellite meetings and workshops:

• Bioinformatics for biodiversity genomics (open meeting)

Kerstin Howe, Sanger, UK

Tyler Alioto, CNAG, Barcelona, Spain

Henrik Lantz, NBIS, Sweden

Discussions

• Clinical long-read sequencing in Sweden (closed meeting)

• Facility forum organized by the BGE consortium (closed meeting)